Analytica chimica acta · 2025
Bottom-up structural analysis of amides by identifying collision-induced dissociation fragment ions: An application in bile acid-amino acid conjugates-targeted sub-metabolome profiling.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Zhou Yuxuan, Li Wei, Niu Xiaoya, Cao Yan, Wang Maodong, He Hangyun, Cao Zheng, Hong Qian, Tu Pengfei, Song Yuelin
Аннотация
BACKGROUND: Amide serves as the key functional group for several chemical families, such as proteins, peptides, amides, and amino acid-conjugated bile acids (AA-BAs), and the featured fragment ions (e.g., a+/-, b+/-, c+/-, x+/-, y+/-, and z+/-) are usually the determinant clues for MS/MS-dependent structural analysis. Here, efforts were made to identify the fragment ions through matching full exciting energy ramp (FEER)-MS3 spectra with authentic amines and/or known substructures, and the justified strategy was thereafter applied to profile AA-BAs-targeted sub-metabolome in biological matrices.
RESULTS: Forty-six amides were collected to configure FEER-MS3 for the featured fragment ions, and FEER-MS2 spectra were acquired for some relevant amines. When amides owned identical substructure, they generated the same FEER-MS3 spectra correlating to the building blocks, and FEER-MS3 spectrum of y+ ion for a given amide matched exactly with FEER-MS2 of the pioneer amine. To assist structural analysis of AA-BAs, 112 authentic compounds were obtained by integrating commercial collection, synthesis, and biotransformation. Through matching FEER-MS3 spectra with FEER-MS3 of known substructures or FEER-MS2 of authentic amines, 85 AA-BAs were identified from a pooled biological sample. Quantitative sub-metabolome comparison involving all detected AA-BAs was conducted between healthy and CCl4-induced liver injury rats. Ala-CA, Leu-CA, Trp-CA, Tyr-CA, Phe-CA, together with Ile-UCA isomer served as the primary differential AA-BA species.
SIGNIFICANCE AND NOVELTY: Differential AA-BAs bearing confirmative identities were disclosed to discriminate liver injury rats from healthy animals. More importantly, a meaningful bottom-up structural analysis strategy that deciphers the featured fragment ions via matching FEER-MSn (n = 2 or 3) with authentic pioneers or known substructures was constructed for amides as well as other chemical families.
RESULTS: Forty-six amides were collected to configure FEER-MS3 for the featured fragment ions, and FEER-MS2 spectra were acquired for some relevant amines. When amides owned identical substructure, they generated the same FEER-MS3 spectra correlating to the building blocks, and FEER-MS3 spectrum of y+ ion for a given amide matched exactly with FEER-MS2 of the pioneer amine. To assist structural analysis of AA-BAs, 112 authentic compounds were obtained by integrating commercial collection, synthesis, and biotransformation. Through matching FEER-MS3 spectra with FEER-MS3 of known substructures or FEER-MS2 of authentic amines, 85 AA-BAs were identified from a pooled biological sample. Quantitative sub-metabolome comparison involving all detected AA-BAs was conducted between healthy and CCl4-induced liver injury rats. Ala-CA, Leu-CA, Trp-CA, Tyr-CA, Phe-CA, together with Ile-UCA isomer served as the primary differential AA-BA species.
SIGNIFICANCE AND NOVELTY: Differential AA-BAs bearing confirmative identities were disclosed to discriminate liver injury rats from healthy animals. More importantly, a meaningful bottom-up structural analysis strategy that deciphers the featured fragment ions via matching FEER-MSn (n = 2 or 3) with authentic pioneers or known substructures was constructed for amides as well as other chemical families.