Fertility and sterility · 2000
Periovulatory human oocytes, cumulus cells, and ovarian leukocytes express type 1 but not type 2 11beta-hydroxysteroid dehydrogenase RNA.
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Smith M P, Mathur R S, Keay S D, Hall L, Hull M G, Jenkins J M
Аннотация
OBJECTIVE: To further elucidate cortisol metabolism in the follicular microenvironment at the time of oocyte retrieval, the presence of 11beta-hydroxysteroid dehydrogenase (HSD) messenger (m)RNA transcripts in oocytes; cumulus cells; granulosa cells; and CD45(+), CD15(+) leukocytes was assessed semiquantitatively.
DESIGN: Controlled study using semiquantitative assessment of 11beta-HSD mRNA.
SETTING: University IVF center.
PATIENT(S): Twenty-six patients undergoing controlled ovarian hyperstimulation for assisted conception.
INTERVENTION(S): Metaphase II oocytes; cumulus cells; granulosa cells, and CD45(+), CD15(+) leukocytes from individual follicular fluid aspirates.
MAIN OUTCOME MEASURES: Semiquantitative analysis of PCR products after total RNA extraction and complementary DNA synthesis.
RESULT(S): Periovulatory human oocytes; cumulus cells; CD45(+), CD15(+) leukocytes; and granulosa cells consistently express type 1 but not type 2 11beta-HSD mRNA. Expression of mRNA is greatest in cumulus cells. Type 1 11beta-HSD mRNA expression varies considerably in all cell types and among individual follicles and patients.
CONCLUSION(S): These studies of mRNA expression suggest that the enzymes present both in and around the periovulatory oocyte will favor a high-cortisol environment.
DESIGN: Controlled study using semiquantitative assessment of 11beta-HSD mRNA.
SETTING: University IVF center.
PATIENT(S): Twenty-six patients undergoing controlled ovarian hyperstimulation for assisted conception.
INTERVENTION(S): Metaphase II oocytes; cumulus cells; granulosa cells, and CD45(+), CD15(+) leukocytes from individual follicular fluid aspirates.
MAIN OUTCOME MEASURES: Semiquantitative analysis of PCR products after total RNA extraction and complementary DNA synthesis.
RESULT(S): Periovulatory human oocytes; cumulus cells; CD45(+), CD15(+) leukocytes; and granulosa cells consistently express type 1 but not type 2 11beta-HSD mRNA. Expression of mRNA is greatest in cumulus cells. Type 1 11beta-HSD mRNA expression varies considerably in all cell types and among individual follicles and patients.
CONCLUSION(S): These studies of mRNA expression suggest that the enzymes present both in and around the periovulatory oocyte will favor a high-cortisol environment.
Предметные рубрики MeSH
11-beta-Hydroxysteroid DehydrogenasesFemaleFertilization in VitroGene Expression RegulationGlyceraldehyde-3-Phosphate DehydrogenasesGranulosa CellsHumansHydroxysteroid DehydrogenasesIsoenzymesLeukocyte Common AntigensLeukocytesLewis X AntigenOocytesOvaryOvulationPlacentaPredictive Value of TestsPregnancyProtein Tyrosine Phosphatase, Non-Receptor Type 1RNA, MessengerTreatment Outcome