A wearable and wireless electrochemical device based on Cu-BTC MOF for pyocyanin detection and wound healing.

The prevalent and serious antimicrobial resistance in Pseudomonas aeruginosa (PA) infections has emerged as a critical public health challenge worldwide. Pyocyanin (PYO), an important virulence component produced by PA, is considered as an ideal indicator for identifying infections linked to this pathogen. This study evaluated ten metal-organic frameworks (MOFs) as signal amplifiers for the sensitive detection of PYO. The findings reveal that electrodes modified with Cu-MOFs exhibited a notable enhancement in current for PYO reduction. Particularly, Cu-1,3,5-benzene tricarboxylic acid (Cu-BTC) demonstrated superior enhancement for PYO reduction, primarily due to its smaller particle size and larger availability of active copper sites. Building on these findings, an innovative wearable device using Cu-BTC was developed to facilitate the wireless electrochemical evaluation of PYO in wounds artificially produced in Sprague-Dawley rat models. The findings indicate that this biocompatible device not only possesses exceptional sensitivity for detecting PYO with a limit of detection (LOD) of 93.5 pM with remarkable stability and selectivity but also shows antimicrobial properties that aid in the wound healing process.