Beyond the surface: Consequences of methyl tert-butyl ether (MTBE) exposure on oxidative stress, haematology, genotoxicity, and histopathology in rainbow trout.

Concerns have been raised about the possible environmental effects of methyl tert-butyl ether (MTBE), which is widely used as a gasoline additive. This research aimed to look at the consequences of MTBE contamination on rainbow trout (Oncorhynchus mykiss), emphasizing oxidative stress, genotoxicity, and histopathological damage. After determining the LC50-96 h value, the effects of sub-lethal doses of MTBE (0 (control), 90, 180, and 450 ppm) on rainbow trout were investigated. In fish tissues, the levels of oxidative stress indicators such as malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. The comet assay, which measures DNA damage in erythrocytes, was used to determine genotoxicity. Histopathological examinations were done on liver and gill tissues to examine potential structural anomalies. The results of this study show that MTBE exposure caused considerable alterations in rainbow trout. Increased oxidative stress was demonstrated by elevated MDA levels and decreased SOD activity, while the comet assay revealed dose-dependent DNA damage, implying genotoxic effects. Histopathological study revealed liver and gill tissue abnormalities, including cell degeneration, necrosis, and inflammation. Overall, this research highlights the possible sub-lethal effects of MTBE contamination on rainbow trout, stressing the need of resolving this issue. Future research should look at the impacts of chronic MTBE exposure and the possibility of bioaccumulation in fish populations.