PloS one · 2026
Reconstruction of a segmental bone defect using vascularized tissue-engineered bone substitutes: An animal study.
СтатьяНазвание и аннотация: Перевод в очереди
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Wang Yulei, Lv Qian, Zhang Xu, Liang Jinlong, Feng Fanzhe, Li Jingyuan, Cui Yi
Аннотация
BACKGROUND: Repairing large segmental bone defects remains a major challenge in orthopedics, with conventional autografting and allografting limited by donor shortages and high complication rates. Tissue-engineered bone substitutes have emerged as a potential solution. This study aimed to assess the vascular regeneration of dual-vascularized tissue-engineered bone substitutes in the reconstruction of large segmental bone defects in rabbits.
METHOD: Demineralized bone matrix (DBM) was used as a scaffold, with endothelial progenitor cells (EPCs) seeded onto it. A vascular channel was created within the DBM-EPCs composite scaffold, and the radial artery was implanted into this channel. New Zealand white rabbits were used to create a 15-mm critical-sized rabbit radial bone defect model, with animals divided into four groups: DBM, DBM+EPCs, DBM+Vascular Bundle,and DBM+EPCs+Vascular Bundle (n = 9 per group).X-ray examinations,gross morphological observations,and CD31 immunofluorescence staining were conducted at 4, 8,and 12 weeks post-surgery. Micro-CT was used to reconstruct the three-dimensional structures of the defects after 12 weeks.
RESULTS: The DBM+EPCs+Vascular Bundle group demonstrated the most significant bone regeneration and vascularization across all time points.X-ray,gross morphology, Micro-CT analysis,HE staining,and CD31 immunofluorescence staining all revealed superior bone regeneration and vascular density in this group compared to the others.
CONCLUSIONS: In conclusion, the dual vascularization strategy significantly enhanced bone regeneration and angiogenesis in the reconstruction of large bone defects. This approach has potential clinical applications for repairing critical-sized bone defects, particularly in anatomical regions with multiple arterial supplies such as the upper limbs and lower legs.
METHOD: Demineralized bone matrix (DBM) was used as a scaffold, with endothelial progenitor cells (EPCs) seeded onto it. A vascular channel was created within the DBM-EPCs composite scaffold, and the radial artery was implanted into this channel. New Zealand white rabbits were used to create a 15-mm critical-sized rabbit radial bone defect model, with animals divided into four groups: DBM, DBM+EPCs, DBM+Vascular Bundle,and DBM+EPCs+Vascular Bundle (n = 9 per group).X-ray examinations,gross morphological observations,and CD31 immunofluorescence staining were conducted at 4, 8,and 12 weeks post-surgery. Micro-CT was used to reconstruct the three-dimensional structures of the defects after 12 weeks.
RESULTS: The DBM+EPCs+Vascular Bundle group demonstrated the most significant bone regeneration and vascularization across all time points.X-ray,gross morphology, Micro-CT analysis,HE staining,and CD31 immunofluorescence staining all revealed superior bone regeneration and vascular density in this group compared to the others.
CONCLUSIONS: In conclusion, the dual vascularization strategy significantly enhanced bone regeneration and angiogenesis in the reconstruction of large bone defects. This approach has potential clinical applications for repairing critical-sized bone defects, particularly in anatomical regions with multiple arterial supplies such as the upper limbs and lower legs.