Journal of ethnopharmacology · 2026
Anti-inflammatory and analgesic activities of the dark glossy green leafy variety of Lasianthera africana: UHPLC-HR-ESI-MS analyses.
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Udoh Blessing I, Essien Emmanuel E, Antia Bassey S, Thomas Paul S, De Leo Marinella
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: The dark glossy green leafy variety of Lasianthera africana P. Beauv. is mostly utilized for its medicinal value and less frequently as vegetable in soup because of its bitter taste. The ethanol and hot aqueous extracts of this dark leafy variety are employed in ethnomedicine for the treatment of stomach ache, pains, inflammatory disorder, among others, but limited studies on chemical and biological properties of this variety are reported in the literature.
AIM OF THE STUDY: The phytochemical composition, the in vivo anti-inflammatory and analgesic activities of the ethanol and hot aqueous extracts of the dark glossy leafy variety of L. africana were determined in this study.
MATERIALS AND METHODS: The chemical compositions of both extracts were characterized by ultra-high performance liquid chromatography coupled with high resolution electrospray ionization mass spectrometer (UHPLC-HR-ESI-MS). The anti-inflammatory and analgesic activities of the extracts were assessed in mice models after the determination of median lethal dose of the extracts.
RESULTS: The UHPLC-HR-ESI-MS analyses revealed the presence of terpenoids and phenols, including hydroxycinnamic acid derivatives and flavonoids. The two extracts shared almost all components, mainly quinic acid derivatives, being caffeoylquinic acids the most abundant in both extracts. The median lethal doses (LD50) of the ethanol and aqueous extracts were 4740 mg/kg and 5000 mg/kg b.w., respectively. The extracts demonstrated good anti-inflammatory effects (45-96% reduction of inflammation) in the xylene-induced oedema in mice and analgesic activities (10.79-24.5 s latent time of pain) in the thermal-induced writhing in mice.
CONCLUSIONS: The inflammatory and analgesic activities of these polar leaves extracts supported the ethnomedicinal value of the dark glossy green leafy variety of L. africana. The UHPLC-HR-ESI-MS analysis and anti-inflammatory properties of the leaves extracts of L. africana are reported for the first time.
AIM OF THE STUDY: The phytochemical composition, the in vivo anti-inflammatory and analgesic activities of the ethanol and hot aqueous extracts of the dark glossy leafy variety of L. africana were determined in this study.
MATERIALS AND METHODS: The chemical compositions of both extracts were characterized by ultra-high performance liquid chromatography coupled with high resolution electrospray ionization mass spectrometer (UHPLC-HR-ESI-MS). The anti-inflammatory and analgesic activities of the extracts were assessed in mice models after the determination of median lethal dose of the extracts.
RESULTS: The UHPLC-HR-ESI-MS analyses revealed the presence of terpenoids and phenols, including hydroxycinnamic acid derivatives and flavonoids. The two extracts shared almost all components, mainly quinic acid derivatives, being caffeoylquinic acids the most abundant in both extracts. The median lethal doses (LD50) of the ethanol and aqueous extracts were 4740 mg/kg and 5000 mg/kg b.w., respectively. The extracts demonstrated good anti-inflammatory effects (45-96% reduction of inflammation) in the xylene-induced oedema in mice and analgesic activities (10.79-24.5 s latent time of pain) in the thermal-induced writhing in mice.
CONCLUSIONS: The inflammatory and analgesic activities of these polar leaves extracts supported the ethnomedicinal value of the dark glossy green leafy variety of L. africana. The UHPLC-HR-ESI-MS analysis and anti-inflammatory properties of the leaves extracts of L. africana are reported for the first time.