Asian Pacific journal of tropical medicine · 2014
Effect of RSCs combined with COP-1 on optic nerve damage in glaucoma rat model.
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Fu Wei-Cai, Jiang Yan, Zhang Lin
Аннотация
OBJECTIVE: To explore effect of retinal stem cells (RSCs) combined with copolymer-1 (COP-1) immunotherapy on optic nerve damage in glaucoma rat model.
METHODS: A total of 40 SD rats were selected for glaucoma model and were randomly divided into 4 groups to observe protective effects of RSCs transplantation combined with COP-1.
RESULTS: Brain-derived neurotrophic factor (BDNF) and insulin like growth factor-1 (IGF-1) were either positive in retina of RSCs transplanted or COP-1 immunological treated rat. Positive rate of BDNF and IGF-1 and expression of mRNA and protein were significantly higher in RSCs transplantation combined with COP-1 immunotherapy treated rats compared with the other 3 groups, in which amount of apoptotic RGCs was lowest.
CONCLUSIONS: RSCs transplantation combined with COP-1 immunotherapy can promote the secretion of BDNF and IGF-1. They protect RGCs in glaucoma rats in coordination, significantly reduce the number of apoptosis RGCs so as to alleviate the optic nerve damage. It ponits a new research direction for treatment of glaucoma.
METHODS: A total of 40 SD rats were selected for glaucoma model and were randomly divided into 4 groups to observe protective effects of RSCs transplantation combined with COP-1.
RESULTS: Brain-derived neurotrophic factor (BDNF) and insulin like growth factor-1 (IGF-1) were either positive in retina of RSCs transplanted or COP-1 immunological treated rat. Positive rate of BDNF and IGF-1 and expression of mRNA and protein were significantly higher in RSCs transplantation combined with COP-1 immunotherapy treated rats compared with the other 3 groups, in which amount of apoptotic RGCs was lowest.
CONCLUSIONS: RSCs transplantation combined with COP-1 immunotherapy can promote the secretion of BDNF and IGF-1. They protect RGCs in glaucoma rats in coordination, significantly reduce the number of apoptosis RGCs so as to alleviate the optic nerve damage. It ponits a new research direction for treatment of glaucoma.
Предметные рубрики MeSH
AnimalsApoptosisBrain-Derived Neurotrophic FactorCells, CulturedChi-Square DistributionDisease Models, AnimalFemaleGlatiramer AcetateGlaucomaInsulin-Like Growth Factor IMaleMicroscopy, FluorescenceOptic Nerve InjuriesPeptidesRNA, MessengerRatsRats, Sprague-DawleyRetinaStem Cell TransplantationStem Cells