Establishment of reference interval for platelet aggregation test with three agonists on automated haemostasis analyzers.

To establish reference intervals (RIs) for the maximal aggregation rate (MA%) mainly of platelet aggregation tests induced by Collagen (COL), epinephrine (EPI), and ristocetin (RIS) using two automated haemostasis analyzers. Platelet aggregation tests were performed on 130 volunteers using CN6000 and CS5100 haemostasis analyzers. RIs were determined by a non-parametric method according to Clinical and Laboratory Standards Institute (CLSI) document EP28-A3c. The coefficient of variation (CV%) of MA% (excluding 0.6 mg/mL RIS) ranged from 3.23% to 6.29%. No significant differences in MA% were observed across gender and age groups. Significant differences were noted between the two analyzers. The RIs induced by COL at 2 µg/mL and 5 µg/mL ranged from 83.4% to 97.5% and 82.0% to 96.9% on the CN6000, and 76.3%-94.4% and 77.1%-95.7% on the CS5100, respectively. For EPI at 5 µmol/L the RIs were 76.0%-94.6% and 70.2%-93.5%. For RIS at 1.2 mg/mL, the RIs were 77.8%-95.0% and 76.4%-91.3%; for RIS at 0.6 mg/mL, were from 0%-3.0% and 0%-3.8% for CN6000 and CS5100, respectively. RIs for MA% induced by COL, EPI and RIS were established using two automated haemostasis analyzers, providing a valuable resource for clinical laboratories.