A novel method for the rapid determination of phenolic compounds based on the nanozyme with laccase-like activity.

Phenolic compounds are prevalent in domestic and industrial effluents, leading a serious environmental hazard. Paper-based analysis device mediated by nanozymes has shown great potential in portable visual determination of phenolic compounds in the environment. In this work, we used nicotinic acid derivatives such as pyridine-2,3-dicarboxylic acid, 2-methylnicotinic acid and 2-aminonicotinic acid by coordinating copper (II) acetate monohydrate coordination to obtain Cu2-COOHNA, Cu2-CH3NA, Cu2-ANA nanozymes with laccase-activity. Compared with natural laccase and CuNA, Cu2-ANA exhibited higher catalytic activity with a similar Km of 0.05 mM and a higher Vmax of 6.08 μM min-1. Cu2-ANA also exhibited a remarkable level of stability and can be used under a wide range of conditions. In addition, Cu2-ANA catalyzed the oxidation of different common phenolic compounds (PCs) and mix PCs. A visually inspective portable sensor constructed by using paper test strips coated with Cu2-ANA employed the colorimetric method for evaluating the concentration of PCs. There is no need for complicated instrumentation, and the popularity and portability of smartphones make on-site testing more efficient and convenient.