Wei sheng yan jiu = Journal of hygiene research · 2004
[Using the FRTL-5 cell to screen the thyroxine disrupting effects of the two pesticides-ethylenethiourea and pendimethalin].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Pan Hongmei, Sun Ding, Wang Jintao, Wu Desheng
Аннотация
OBJECTIVE: In order to study the effects of ethylenethiourea(ETU) and pendimethalin on synthesis and secretion of thyroglobulin(Tg) in FRTL-5 cells in vitro.
METHODS: MTT test was used to investigate the cytotoxicity of the two chemicals to FRTL-5 cell. When the concentration of pendimethalin exceeded 5 ng/microliter, and the concentration of ethylenethiourea exceeded 360 ng/microliter, there was distinct cytotoxicity to FRTL-5 cell. The radioimmunoassay was also used to detect the level of thyroglobulin in culture. The exposure doses in radioimmunoassay was based on the MTT test.
RESULTS: The concentration of Tg was decreased in medium and high dose of pendimethalin and ethylenethiourea.
CONCLUSION: It is likely, therefore, that the two pesticides might disrupt FRTL-5 cell of synthesizing and secreting Tg.
METHODS: MTT test was used to investigate the cytotoxicity of the two chemicals to FRTL-5 cell. When the concentration of pendimethalin exceeded 5 ng/microliter, and the concentration of ethylenethiourea exceeded 360 ng/microliter, there was distinct cytotoxicity to FRTL-5 cell. The radioimmunoassay was also used to detect the level of thyroglobulin in culture. The exposure doses in radioimmunoassay was based on the MTT test.
RESULTS: The concentration of Tg was decreased in medium and high dose of pendimethalin and ethylenethiourea.
CONCLUSION: It is likely, therefore, that the two pesticides might disrupt FRTL-5 cell of synthesizing and secreting Tg.