Science (New York, N.Y.) · 2024
The landscape of RNA binding proteins in mammalian spermatogenesis.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Li Yang, Wang Yuanyuan, Tan Yue-Qiu, Yue Qiuling, Guo Yueshuai, Yan Ruoyu, Meng Lanlan, Zhai Huicong, Tong Lingxiu, Yuan Zihan, Li Wu, Wang Cuicui, Han Shenglin, Ren Sen, Yan Yitong, Wang Weixu, Gao Lei, Tan Chen, Hu Tongyao, Zhang Hao, Liu Liya, Yang Pinglan, Jiang Wanyin, Ye Yiting, Tan Huanhuan, Wang Yanfeng, Lu Chenyu, Li Xin, Xie Jie, Yuan Gege, Cui Yiqiang, Shen Bin, Wang Cheng, Guan Yichun, Li Wei, Shi Qinghua, Lin Ge, Ni Ting, Sun Zheng, Ye Lan, Vourekas Anastasios, Guo Xuejiang, Lin Mingyan, Zheng Ke
Аннотация
Despite continuous expansion of the RNA binding protein (RBP) world, there is a lack of systematic understanding of RBPs in the mammalian testis, which harbors one of the most complex tissue transcriptomes. We adapted RNA interactome capture to mouse male germ cells, building an RBP atlas characterized by multiple layers of dynamics along spermatogenesis. Trapping of RNA-cross-linked peptides showed that the glutamic acid-arginine (ER) patch, a residue-coevolved polyampholytic element present in coiled coils, enhances RNA binding of its host RBPs. Deletion of this element in NONO (non-POU domain-containing octamer-binding protein) led to a defective mitosis-to-meiosis transition due to compromised NONO-RNA interactions. Whole-exome sequencing of over 1000 infertile men revealed a prominent role of RBPs in the human genetic architecture of male infertility and identified risk ER patch variants.