[Measurement of acetonitrile in blood and urine by head-space gas chromatography].

OBJECTIVE

To establish the method for measurement of acetonitrile in blood and urine by head-space gas chromatography.

METHODS

DB-ALC1 (30 m x 320 microm x 1.8 microm) and DB-ALC2 (30 m x 320 microm x 1.2 microm) capillary column were used to measure the acetonitrile in blood and urine with the isopropanol as internal standard reference.

RESULTS

The limits of detection of acetonitrile in both blood and urine were 0.5 microg/mL, with a linear range of 5-1000 microg/mL (r = 0.999).The accuracy of this method was 93.2%-98.0%. The RSD for the intra-day and inter-day were less than 3.7%.

CONCLUSION

The method is capable for measurement analysis of acetonitrile in blood and urine.