Fa yi xue za zhi · 2006
[Study of Papaver somniferum cultivars identification by TD-RAPD technique].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
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Lu Fan, Hong Jun-Yan, He Rui, Li Li-Sha
Аннотация
OBJECTIVE: The feasibility of Papaver somniferum L. cultivars identification was explored by TD-RAPD technique.
METHOD: Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method.
RESULT: We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers.
CONCLUSION: TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy.
METHOD: Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method.
RESULT: We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers.
CONCLUSION: TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy.