ESMO gastrointestinal oncology · 2025
Development and characterization of orthotopic patient-derived xenograft models of peritoneal metastatic mucinous appendiceal adenocarcinoma.
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Ito Ichiaki, Pattalachinti Vinay K, Yousef Abdelrahman Mg, Chowdhury Saikat, Fanaeian Mohammad M, Haque Emaan, Gunes Betul Beyza, Yousef Mahmoud, Salle Emma R, Zeineddine Mohammad A, Ji Shuangxi, Li Ruonan, Wang Wenyi, Helmink Beth A, Taggart Melissa W, White Michael G, Fournier Keith F, Fowlkes Natalie W, Shen John Paul
Аннотация
BACKGROUND: Appendiceal adenocarcinomas (AAs) are a rare and heterogeneous group of tumors for which few preclinical models exist. The lack of preclinical models of AA has hindered drug development and is a major factor in why AA remains without a single FDA-approved systemic treatment.
MATERIALS AND METHODS: Tumors from 16 patients with appendiceal neoplasms (15 AA and one HAMN) were implanted into flank and peritoneal cavity of immunodeficient mice leading to the successful establishment of 3 AAPDX models. Histological, immunohistochemical, genetic, and transcriptomic comparison of patient and PDX tumors were performed.
RESULTS: Higher tumor grade, peritoneal implantation, and RAS/RAF mutation were associated with successful tumor engraftment. Comparison of histological, immunohistochemical and molecular analyses including both RNA and DNA sequencing revealed that the PDX models recreate many of the features of metastatic AA, but also displayed several differences between paired PDX and human tumors highlighting the intra-tumoral heterogeneity of AA within each patient. Notably tumor from two patients with primarily low-grade mucinous adenocarcinoma converted to high-grade histology in PDX. Transcriptomic comparison of patient and PDX tumors identified increased Myc and E2F signaling, suggesting that activation of Myc may be a driver of de-differentiation in AA. The established PDX were able to undergo serial passaging and expansion and exhibited stable histological features during this process, allowing for drug testing.
CONCLUSIONS: These molecularly profiled, orthotopic PDX models of metastatic AA represent a unique resource for future exploration to identify novel therapies for this orphan disease.
MATERIALS AND METHODS: Tumors from 16 patients with appendiceal neoplasms (15 AA and one HAMN) were implanted into flank and peritoneal cavity of immunodeficient mice leading to the successful establishment of 3 AAPDX models. Histological, immunohistochemical, genetic, and transcriptomic comparison of patient and PDX tumors were performed.
RESULTS: Higher tumor grade, peritoneal implantation, and RAS/RAF mutation were associated with successful tumor engraftment. Comparison of histological, immunohistochemical and molecular analyses including both RNA and DNA sequencing revealed that the PDX models recreate many of the features of metastatic AA, but also displayed several differences between paired PDX and human tumors highlighting the intra-tumoral heterogeneity of AA within each patient. Notably tumor from two patients with primarily low-grade mucinous adenocarcinoma converted to high-grade histology in PDX. Transcriptomic comparison of patient and PDX tumors identified increased Myc and E2F signaling, suggesting that activation of Myc may be a driver of de-differentiation in AA. The established PDX were able to undergo serial passaging and expansion and exhibited stable histological features during this process, allowing for drug testing.
CONCLUSIONS: These molecularly profiled, orthotopic PDX models of metastatic AA represent a unique resource for future exploration to identify novel therapies for this orphan disease.