Fertility and sterility · 2007
Survivin gene expression in granulosa cells from infertile patients undergoing in vitro fertilization-embryo transfer.
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Fujino Kuniko, Yamashita Yoshiki, Hayashi Atushi, Asano Masako, Morishima Shoko, Ohmichi Masahide
Аннотация
OBJECTIVE: To evaluate survivin gene expression in granulosa cells from infertile patients and examine the relationship between survivin gene expression and infertile clinical background.
DESIGN: Prospective study.
SETTING: IVF-ET program at Osaka Medical College.
PATIENT(S): Twenty-eight patients underwent ovulation induction for IVF-ET performed because of tubal infertility, male factor infertility, or endometriosis.
INTERVENTION(S): Granulosa cells obtained at oocyte retrieval were examined for survivin gene expression by quantitative reverse transcription-polymerase chain reaction.
MAIN OUTCOME MEASURE(S): Hormone environment, number of oocytes, fertilization rate, high-quality embryo rate, pregnancy rate, and expression of survivin genes.
RESULT(S): Survivin gene expression was detected in all granulosa cells. The gene expression levels of survivin in patients with endometriosis were significantly lower than those in patients with male factor infertility. The gene expression levels of survivin in total pregnant patients were higher than those in total nonpregnant patients and than those in the male factor infertility group, and there was no correlation between gene expression level and serum E(2) level.
CONCLUSION(S): Survivin may be used as an indicator of the success of IVF-ET, and the existence of endometriosis may elevate the apoptosis of granulosa cells.
DESIGN: Prospective study.
SETTING: IVF-ET program at Osaka Medical College.
PATIENT(S): Twenty-eight patients underwent ovulation induction for IVF-ET performed because of tubal infertility, male factor infertility, or endometriosis.
INTERVENTION(S): Granulosa cells obtained at oocyte retrieval were examined for survivin gene expression by quantitative reverse transcription-polymerase chain reaction.
MAIN OUTCOME MEASURE(S): Hormone environment, number of oocytes, fertilization rate, high-quality embryo rate, pregnancy rate, and expression of survivin genes.
RESULT(S): Survivin gene expression was detected in all granulosa cells. The gene expression levels of survivin in patients with endometriosis were significantly lower than those in patients with male factor infertility. The gene expression levels of survivin in total pregnant patients were higher than those in total nonpregnant patients and than those in the male factor infertility group, and there was no correlation between gene expression level and serum E(2) level.
CONCLUSION(S): Survivin may be used as an indicator of the success of IVF-ET, and the existence of endometriosis may elevate the apoptosis of granulosa cells.
Предметные рубрики MeSH
AdultApoptosisBiomarkersEmbryo TransferEndometriosisEstradiolFallopian Tube DiseasesFemaleFertilizationFertilization in VitroGene Expression Regulation, DevelopmentalGranulosa CellsHumansInfertilityInfertility, MaleInhibitor of Apoptosis ProteinsMaleMicrotubule-Associated ProteinsNeoplasm ProteinsOocyte RetrievalOvulation InductionPregnancyPregnancy RateProspective StudiesRNA, MessengerReverse Transcriptase Polymerase Chain ReactionSurvivinTreatment Outcome