[Preliminary examination of a LB-H2O2 substance that inhibit Neisseria gonorrhea growth].

OBJECTIVE

To study the possible mechanism of H2O2-producing lactobacilli inhibited neisseria gonorrhoeae growth.

METHODS

H2O2-producing lactobacilli were coincubated with neisseria gonorrhoeae in vitro, LB+ cell were disrupted by sonication, the protein in the lactobacilli lysate was separated by filtration through different MW sizes membranes and was examined for the ability to inhibit N. gonorrhoeae catalase activity.

RESULTS

When coincubation medium was at pH 5.0, there was a significant decrease in the gonococcal catalase activity, the lysates of LB+ also effectively inhibited gonococcal catalase activity. This inhibition was retained upon heating of lysate to 100 degrees C for 15 minutes but was lost with proteinase K treatment.

CONCLUSIONS

The LB+ may inhibit growth of gonococci by production of catalase inhibitors.