BMC urology · 2025
Investigation of mycoplasma and ureaplasma species using a molecular method in male patients suffering from urethritis symptoms: a cross-sectional study in the city of Antalya.
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Karpuz Tuncer, Şambel Murat, Özyurt Özlem Koyuncu, Caylan Ahmet Ender, Kutlu Ömer, Öğünç Meral Dilara, Öngüt Gözde, Özhak Betil, Saygılı Burak, Çolak Dilek, Yazısız Hatice
Аннотация
BACKGROUND: This study aimed to determine whether Mycoplasma (M) genitalium, M. hominis, Ureaplasma (U) urealyticum, and U. parvum were present in male patients with symptoms of urethritis.
METHODS: First-void urine and genital discharge samples were collected from 94 men. The samples were examined for the presence of M. genitalium, M. hominis, U. urealyticum, and U. parvum using a multiplex polymerase chain reaction (PCR) method (BioGX Mycoplasma-Ureaplasma-OSR commercial multiplex PCR kit, BioGX, the Netherlands). The presence of Trichomonas vaginalis and Neisseria (N) gonorrhoeae was investigated using microscopy and culture methods. In the M. genitalium-positive samples, macrolide resistance was evaluated using the Macrolide-R/MG ELITe MGB Kit (ELITechGroup S.p.A., Italy) on the ELITe BeGenius system.
RESULTS: A total of 55 microorganisms were detected in 41.5% of the patients (n = 39; U. urealyticum [n = 18], U. parvum [n = 14], N. gonorrhoeae [n = 9], M. hominis [n = 8], and M. genitalium [n = 6]). The positivity rates of M. genitalium and N. gonorrhoeae were statistically higher in the patients with more than 3 partners in the last 12 months, and those of U. urealyticum and N. gonorrhoeae were higher in the patients with genital discharge (p < 0.05). In addition, a significant relationship was found between N. gonorrhoeae positivity and genital itching and pain/discomfort during sexual intercourse (p < 0.05). Macrolide resistance was detected in 2 (33.3%) of the 6 M. genitalium-positive samples.
CONCLUSION: In this study, U. urealyticum, N. gonorrhoeae, and M. genitalium were detected in 19.1%, 9.6%, and 6.4% of the male patients who presented with symptoms of urethritis, respectively.
METHODS: First-void urine and genital discharge samples were collected from 94 men. The samples were examined for the presence of M. genitalium, M. hominis, U. urealyticum, and U. parvum using a multiplex polymerase chain reaction (PCR) method (BioGX Mycoplasma-Ureaplasma-OSR commercial multiplex PCR kit, BioGX, the Netherlands). The presence of Trichomonas vaginalis and Neisseria (N) gonorrhoeae was investigated using microscopy and culture methods. In the M. genitalium-positive samples, macrolide resistance was evaluated using the Macrolide-R/MG ELITe MGB Kit (ELITechGroup S.p.A., Italy) on the ELITe BeGenius system.
RESULTS: A total of 55 microorganisms were detected in 41.5% of the patients (n = 39; U. urealyticum [n = 18], U. parvum [n = 14], N. gonorrhoeae [n = 9], M. hominis [n = 8], and M. genitalium [n = 6]). The positivity rates of M. genitalium and N. gonorrhoeae were statistically higher in the patients with more than 3 partners in the last 12 months, and those of U. urealyticum and N. gonorrhoeae were higher in the patients with genital discharge (p < 0.05). In addition, a significant relationship was found between N. gonorrhoeae positivity and genital itching and pain/discomfort during sexual intercourse (p < 0.05). Macrolide resistance was detected in 2 (33.3%) of the 6 M. genitalium-positive samples.
CONCLUSION: In this study, U. urealyticum, N. gonorrhoeae, and M. genitalium were detected in 19.1%, 9.6%, and 6.4% of the male patients who presented with symptoms of urethritis, respectively.