Cytogenetic and genome research · 2024
Cytogenomic Characterization of Murine Osteosarcoma Cell Line SEWA.
СтатьяНазвание и аннотация: Перевод в очереди
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Liehr Thomas, Rincic Martina
Аннотация
INTRODUCTION: The SEWA cell line, which is derived from a virus-induced murine osteosarcoma (OS) ascites, was established in the 1980s from a serially transplanted male-derived tumor that was first published in 1961. It has been applied in about 50 studies but was never genetically characterized in detail; this study fills that gap.
METHODS: The SEWA cell line was analyzed for its chromosomal constitution using molecular cytogenetic approaches. Array comparative genomic hybridization was performed to characterize copy number alterations.
RESULTS: SEWA has a near-diploid karyotype without Y-chromosome material. The complex karyotype includes neocentrics and simple and complex rearrangements. Amplification of MYC oncogene was detected in two homogeneously staining regions on two different derivative chromosomes.
CONCLUSION: An in silico translation of the obtained results to the human genome indicated that SEWA is suitable as a model for advanced human OS.
METHODS: The SEWA cell line was analyzed for its chromosomal constitution using molecular cytogenetic approaches. Array comparative genomic hybridization was performed to characterize copy number alterations.
RESULTS: SEWA has a near-diploid karyotype without Y-chromosome material. The complex karyotype includes neocentrics and simple and complex rearrangements. Amplification of MYC oncogene was detected in two homogeneously staining regions on two different derivative chromosomes.
CONCLUSION: An in silico translation of the obtained results to the human genome indicated that SEWA is suitable as a model for advanced human OS.