[An experimental study on compound anisodine III for softening scar of mouse skin after burn].

OBJECTIVE

The aim of this study was to investigate the mechanism of compound anisodine (CA) III which can rapidly soften the cicatrized tissue in clinical practice.

METHODS

2 mol/L NaOH was applied onto the back of 36 mice for one minute to induce deep grade skin burn injury. After 1 week local cicatrization occurred. The mice were randomly divided into 3 groups: 1 ml saline was used in group I as control, hyaluronidase 150 U dissolved in 1 ml 2% procaine was used in group II and 0.005% CA 0.1 ml in 2% procaine 1 ml with hyaluronidase 150 U was used in group III. Subcutaneous injection once every other day for two weeks was the method of drug administration in all the 3 groups and then all the rats were euthanized. The burned skin was sectioned for HE and modified Masson's stains.

RESULTS

Histopathology revealed there were epidermis proliferation, keratinization and fibrosis with disorderly arranged collagenous fibers invading into muscles and a large amount of inflammatory cells infiltrating the lesion in groups I and II. However, the skin was nearly normal in group III.

CONCLUSIONS

CA III can inhibit the epidermic and collagenous proliferation, improve the tissue blood supply and have antiinflammatory effect to prevent scar formation and promote epidermic repair in the skin.