Acta chirurgica Belgica · 2017
Seeding nerve sutures with minced nerve-graft (MINE-G): a simple method to improve nerve regeneration in rats.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
D'Arpa Salvatore, Zabbia Giovanni, Cannizzaro Carla, Salimbeni Grazia, Plescia Fulvio, Mariolo Alessio Vincenzo, Cassata Giovanni, Cicero Luca, Puleio Roberto, Martorana Anna, Moschella Francesco, Cordova Adriana
Аннотация
BACKGROUND: The aim of this study was to assess the effect of seeding the distal nerve suture with nerve fragments in rats.
METHODS: On 20 rats, a 15 mm sciatic nerve defect was reconstructed with a nerve autograft. In the Study Group (10 rats), a minced 1 mm nerve segment was seeded around the nerve suture. In the Control Group (10 rats), a nerve graft alone was used. At 4 and 12 weeks, a walking track analysis with open field test (WTA), hystomorphometry (number of myelinated fibers (n), fiber density (FD) and fiber area (FA) and soleus and gastrocnemius muscle weight ratios (MWR) were evaluated. The Student t-test was used for statistical analysis.
RESULTS: At 4 and 12 weeks the Study Group had a significantly higher n and FD (p = .043 and .033). The SMWR was significantly higher in the Study Group at 12 weeks (p = .0207).
CONCLUSIONS: Seeding the distal nerve suture with nerve fragments increases the number of myelinated fibers, the FD and the SMWR. The technique seems promising and deserves further investigation to clarify the mechanisms involved and its functional effects.
METHODS: On 20 rats, a 15 mm sciatic nerve defect was reconstructed with a nerve autograft. In the Study Group (10 rats), a minced 1 mm nerve segment was seeded around the nerve suture. In the Control Group (10 rats), a nerve graft alone was used. At 4 and 12 weeks, a walking track analysis with open field test (WTA), hystomorphometry (number of myelinated fibers (n), fiber density (FD) and fiber area (FA) and soleus and gastrocnemius muscle weight ratios (MWR) were evaluated. The Student t-test was used for statistical analysis.
RESULTS: At 4 and 12 weeks the Study Group had a significantly higher n and FD (p = .043 and .033). The SMWR was significantly higher in the Study Group at 12 weeks (p = .0207).
CONCLUSIONS: Seeding the distal nerve suture with nerve fragments increases the number of myelinated fibers, the FD and the SMWR. The technique seems promising and deserves further investigation to clarify the mechanisms involved and its functional effects.
Предметные рубрики MeSH
Analysis of VarianceAnimalsDisease Models, AnimalGraft SurvivalImage Interpretation, Computer-AssistedNerve RegenerationNeurosurgical ProceduresPeripheral Nerve InjuriesRandom AllocationRatsRats, Sprague-DawleyRisk FactorsSciatic NerveSuture TechniquesTissue TransplantationTissue and Organ HarvestingTransplantation, Autologous