POTENTIAL RISKS OF THE SPREAD OF ANTIBIOTIC-RESISTANT MICROORGANISMS AND ANTIBIOTIC-RESISTANCE GENES IN POTABLE WATER - HUMAN ORGANISM CHAIN.

OBJECTIVE

The aim: Determination of circulation interrelations between antibiotic-resistant microorganisms of Enterobacteriaceae family and their resistance genes in clinical strains and potable water samples taken in Uzhhorod and Uzhhorod district.

PATIENTS AND

METHODS

Materials and methods: We carried out generic identification of the microorganisms isolated from clinical samples of the oral cavity of 64 patients suffering from periodontal inflammatory diseases, and potable water samples taken from sources of public centralized and decentralized water supply; the isolated microorganisms were tested for antibiotic sensitivity by the Kirby-Bauer disc diffusion method according to EUCAST. With the help of molecular-genetic methods, the total DNA of potable water was isolated and tested for the presence of the following genetic resistance determinants: carbapenems blaNDM; blaOXA48-like; tetracyclines blaTet-M; cephalosporins blaCTX-M.

RESULTS

Results

In the microbiota of the clinical material and potable water samples, the same spectrum of microorganisms belonging to Enterobacteriaceae family dominated; the isolated bacteria showed a high resistance level to beta-lactam antibiotics and to natural antibiotic preparations. The highest level of resistance was established for microorganisms isolated from well water samples, where genetic resistance determinants to blaCTX-M cephalosporins and blaTet tetracyclins were also revealed.

CONCLUSION

Conclusions

The obtained results proved high probability of the spread of antibiotic-resistant microorganisms and their genetic resistance determinants via potable water.