[Tumor cells lysates pulsed dendritic cells for immunotherapy of ovarian cancer in rats: in-vitro study].

OBJECTIVE

To study the feasibility of ovarian tumor cells lysates pulsed dendritic cells (DCs) in the immunotherapy of tumor prevention via cytotoxic test in-vitro.

METHODS

Forty rats were randomly divided into 2 groups: experimental and control groups. Ten rats from each group were killed and DCs were isolated and cultured. Frozen-thawed antigen of in the study group, rat's Ovarian cancer cells of the line NuTu-19 were cultured, freeze-melt antigens were added at day 3. Two groups co-cultured with T lymphocytes from the other rat's spleen on the 14th day. Then observed the shape of DCs, contrasted the proliferation and killing rate.

RESULTS

In the study group, DCs could significantly stimulate T lymphocytes multiplication and produce tumor specific cytotoxic T lymphocytes (CTL). When the ratios of stimulating cells (SC) to reaction cells (RC) were 1:3, 1:10, 1:30 and 1:100, the stimulating index (SI) in the study group were much higher than those in control group (P<0.01). When the ratios of SC is the same with RC (SC:RC=3:1, 10:1, 30:1): the killing and wounding rate to tumor cells of study group [(40.9+/-1.2)%, (69.8+/-1.8)%, (89.0+/-0.4%)] is significantly higher than those of control group [(30.9+/-1.9)%, (34.4+/-2.1)%, (51.0+/-1.5)%] (P < 0.01).

CONCLUSION

DC could activate T lymphocytes proliferation and produce CTL to kill and wound tumor cells in-vitro.