Molecular biology reports · 2026
Comparative multi-pathway inhibition of breast cancer by micro-and nano-formulated Curcumin.
СтатьяСравнительное исследованиеНазвание и аннотация: Перевод в очереди
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Moawad Mahmoud, Altrawy Afaf, Nasr Ghada M, Abd El Aal Wael S, Nasr Mohamed Y, Eissa Mohab S, Shaker Emad S
Аннотация
OBJECTIVES AND BACKGROUND: Breast cancer (BC) remains a leading cause of cancer-related death among women. There is an urgent need for new therapies with fewer side effects. This study evaluated the anticancer potential of curcumin micro- and nanocapsules on MCF-7 breast cancer cells and explored the underlying molecular mechanisms using bioinformatics analysis.
MATERIALS AND METHODS: Curcumin micro- and nanocapsules were produced through mechanical encapsulation and tested on MCF-7 cells. Cell viability was measured by MTT assay, apoptosis and cell cycle changes were analyzed by flow cytometry, and gene expression of C-myc, Ki-67, EGFR, VEGFR2, and Caspase-3 was evaluated by real-time PCR. Public datasets (TCGA and CPTAC) were analyzed to identify curcumin-related genes, pathways, and drug-target networks.
RESULTS: Both formulations reduced cell viability by 45-60% and significantly increased apoptosis, with nanocapsules being more potent. Nanocapsules caused G2/M phase arrest and a 3.8-fold rise in Caspase-3 expression, while microcapsules mainly induced G0/G1 arrest. Treatments suppressed the proliferation markers C-myc and Ki-67, along with EGFR and VEGFR2. Bioinformatics analysis confirmed that these genes are key regulators in MAPK, PI3K/Akt, WNT, and HER2 pathways affected by curcumin.
CONCLUSION: Nano-formulated curcumin demonstrates strong, multi-target anticancer activity by blocking cell growth and angiogenesis while promoting apoptosis. These findings support its potential as a safe and effective therapeutic option for breast cancer.
MATERIALS AND METHODS: Curcumin micro- and nanocapsules were produced through mechanical encapsulation and tested on MCF-7 cells. Cell viability was measured by MTT assay, apoptosis and cell cycle changes were analyzed by flow cytometry, and gene expression of C-myc, Ki-67, EGFR, VEGFR2, and Caspase-3 was evaluated by real-time PCR. Public datasets (TCGA and CPTAC) were analyzed to identify curcumin-related genes, pathways, and drug-target networks.
RESULTS: Both formulations reduced cell viability by 45-60% and significantly increased apoptosis, with nanocapsules being more potent. Nanocapsules caused G2/M phase arrest and a 3.8-fold rise in Caspase-3 expression, while microcapsules mainly induced G0/G1 arrest. Treatments suppressed the proliferation markers C-myc and Ki-67, along with EGFR and VEGFR2. Bioinformatics analysis confirmed that these genes are key regulators in MAPK, PI3K/Akt, WNT, and HER2 pathways affected by curcumin.
CONCLUSION: Nano-formulated curcumin demonstrates strong, multi-target anticancer activity by blocking cell growth and angiogenesis while promoting apoptosis. These findings support its potential as a safe and effective therapeutic option for breast cancer.