[Microbiological evaluation of the vagina in women being screened for inflammatory and precancerous conditions of female genitalia].

The aim of this work was to study the microbiology of vaginal secretion in 3665 (19 to 72 year old) women investigated in the screening programme for inflammatory and malignant diseases. Microbiological examinations of the vaginal contents were carried out using direct preparations (Miller and Gram methods) and different media for cultures. Normal biocenosis (I and II grade of cleanliness) of the vagina was observed in 40.74% investigated patients. Abnormal biocenosis was classified as early, advanced and chronic state infections. In our study we present data on isolated strains of saprophytic organisms and pathogens including yeasts and Trichomonas vaginalis in early and chronic infections. There were homogeneous and mixed infections. The role of the physiological Lactobacillus species inhibiting development of pathogenic flora is suggestive of beneficial influence on the vaginal environment. Candidiasis of the vagina was identified in 11.35% examined women. High percentage Candida growth was found together with Lactobacillus vaginalis. It is noteworthy that trichomoniasis was less frequent compared to our previous reports. Totally we were able to isolate 2597 strains of bacteria. Bacteria type of abnormal biocenosis was found in 46.82%. The data from this study indicated that microbiological pictures (stained acc. to both Miller and Gram methods) are still of importance for the evaluation of vaginal contents. The second significant factor in microbiology of human vagina is the role of culture. This role of cultures for microbiologic studies of vaginal contents is beyond any doubt.