Research on the extraction of total saponins from hazel mushrooms via an ultrasound-assisted extraction method and their antitumor activity.

Currently, "medicinal food" is a trending topic of discussion given the various bioactive compounds found within edible mushrooms. However, research on the collection and biological potency of saponins extracted from hazel mushroom, a species of Armillaria, is lacking. We aimed to conduct additional studies with respect to our previous endeavor, which was an effort to determine possible methods that can be used to alleviate ultrasonic-assisted extraction conditions of compounds and determine possible anticancer effects. Response surface methodology can be used to identify good extraction parameters, such as the ethanol concentration 73.28%, solid/liquid ratio 1:24.88 g/mL, and time 12.5 min, to obtain a maximum value of 21.4%. After extraction, we analyzed specific patterns of some saponins via ultrahigh performance liquid chromatography/ quadrupole time-of-flight mass spectrometry, which meant that these compound act on tumor cells. This study was conducted to determine the effect of such compounds on cancer.