Efficient enrichment of the therapeutic lead JBIR-15 from fermentation broth of the marine-derived strain Aspergillus sp. L14-OE::laeA2 using macroporous adsorption resin.

JBIR-15 as a therapeutic agent was one of the major cyclolipopeptides produced by a marine-derived mutant Aspergillus sp. L14-OE::laeA2. In order to develop an efficient and green extraction process for JBIR-15 enrichment from its fermentation broth, a total of 12 macroporous adsorption resins were evaluated according to their adsorption rates and desorption capacities. The results showed that LX-1600 possessed the best adsorption rate of 97.7% and the best desorption capacity up to 37.2 mg/g, and the adsorption behavior of JBIR-15 conformed to the pseudo-second-order kinetics and the Langmuir isotherm model. Dynamic adsorption/desorption experiments using LX-1600 resin column indicated that the optimal adsorption parameters were determined as 134 BV of fermentation broth with 0.21 mg/mL of JBIR-15 and a flow rate of 8 BV/h and the best desorption conditions were confirmed as follows: gradient elution with 8 BV of water, 8 BV of 10% (v/v) ethanol, 8 BV of 20% (v/v) ethanol and 8 BV of 50% (v/v) ethanol in sequence at 3 BV/h. After one run of adsorption and desorption, the content of the target substance JBIR-15 in the final product increased from 13.6% to 45.2% with recovery rate of 96.9%, suggesting its potential application in large-scale production of JBIR-15.