Cell death assays for the detection of acute kidney tubular necrosis.

Acute tubular necrosis (ATN) is a central driver of nephron loss and progression to kidney failure. Dissecting the cellular pathways that govern tubular necrosis requires reliable systems to model and quantify cell death. This chapter introduces methodologies for isolating murine renal tubules ex vivo and describes a panel of assays-including biochemical, molecular, and imaging approaches-that enable the detection of regulated necrosis in kidney tubules. We provide theoretical background, practical workflows, examples of applications.