In vivo (Athens, Greece) · 2012
The zebrafish--Danio rerio--is a useful model for measuring the effects of small-molecule mitigators of late effects of ionizing irradiation.
СтатьяПоддержка NIH: внешняя программаНазвание и аннотация: Перевод в очереди
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Epperly Michael W, Bahary Nathan, Quader Mubina, Dewald Valerie, Greenberger Joel S
Аннотация
BACKGROUND/AIM: Use of zebrafish models may decrease the cost of screening new irradiation protectors and mitigators.
MATERIALS AND METHODS: Zebrafish (Danio rerio) models were tested for screening water-soluble radiation protectors and mitigators. Irradiation of embryos and monitoring survival, and measuring fibrosis of the caudal musculature of adults allowed for testing of acute and late effects, respectively.
RESULTS: Incubation of zebrafish embryos either before or after irradiation in ethyl pyruvate (1 mM) increased survival. Irradiation of adults to 15 to 75 Gy, delivered in single-fraction at 13 Gy/min, showed dose-dependent fibrosis at 30 days, quantitated as physiological decrease in swimming tail movement, and histopathological detection of collagen deposition in the dorsal musculature. Continuous administration of small-molecule radioprotector drugs in the water after irradiation reduced both acute and chronic injuries.
CONCLUSION: The zebrafish is cost-effective for screening new radiation countermeasures.
MATERIALS AND METHODS: Zebrafish (Danio rerio) models were tested for screening water-soluble radiation protectors and mitigators. Irradiation of embryos and monitoring survival, and measuring fibrosis of the caudal musculature of adults allowed for testing of acute and late effects, respectively.
RESULTS: Incubation of zebrafish embryos either before or after irradiation in ethyl pyruvate (1 mM) increased survival. Irradiation of adults to 15 to 75 Gy, delivered in single-fraction at 13 Gy/min, showed dose-dependent fibrosis at 30 days, quantitated as physiological decrease in swimming tail movement, and histopathological detection of collagen deposition in the dorsal musculature. Continuous administration of small-molecule radioprotector drugs in the water after irradiation reduced both acute and chronic injuries.
CONCLUSION: The zebrafish is cost-effective for screening new radiation countermeasures.