Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) · 2025
Genotoxic and cytotoxic effect of vanadium inhalation and oral administration of Hintonia latiflora methanolic extract in mice.
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Cervantes-Valencia María Eugenia, Rojas-Lemus Marcela, López-Valdez Nelly, González-Villalva Adriana, Fortoul Teresa I
Аннотация
INTRODUCTION: Environmental air pollution constitutes a significant problem for large cities, primarily due to anthropogenic activities such as burning fossil fuels, which have led to an escalation that release suspended particulate matter (PM);vanadium, a metal adhered to PM, leading to their entry into the bloodstream and subsequently V distribution to various organs. Research has demonstrated that exposure to vanadium (V) can induce single-strand breaks and the presence of micronuclei in cells. In addition to this, the use of herbal remedies has seen a marked increase on a global scale in recent years. It is estimated that more population makes use of these remedies without being aware of their potential health implications. Hintonia latiflora (Hl) is a herbal remedy commonly used, reported also as genotoxic.
OBJECTIVE: To evaluate the genotoxic and cytotoxic effect of V inhalation and co-exposure with the administration of methanolic extract of Hl (HlMeOHe).
METHOD: Twenty-five male CD1 mice distributed in four groups were used: control, V, Tween 80 % (0.04 %), HlMeOHe (250 mg/kg per day) and V+HlMeOHe (250 mg/kg per day). Animals inhaled V twice a week. Blood samples were obtained at designated time points: 0, 24 and 72 hours; 7, 14 and 21 days. Subsequently, the samples were subjected to rigorous testing procedures, including the assessment of cell viability by fluorochromes, alkaline comet assay and micronucleus assay with acridine orange.
RESULTS: The inhalation of V, either as a standalone treatment or in conjunction with HlMeOHe, did not affect cell viability. Administration of HlMeOHe, did not result in the generation of single-strand breaks, a finding that contrast with the effects of V. The co-exposure of V and HlMeOHe exhibited from day 14-21 a significant decrease (p ≤ 0.05) in micronuclei assay, as well as a in single-strand breaks (p < 0.05) compared against control group.
CONCLUSION: These results suggest that HlMeOHe is not cytotoxic or genotoxic maybe because of its phenolic compounds, reducing the genotoxic effect induced by V.
OBJECTIVE: To evaluate the genotoxic and cytotoxic effect of V inhalation and co-exposure with the administration of methanolic extract of Hl (HlMeOHe).
METHOD: Twenty-five male CD1 mice distributed in four groups were used: control, V, Tween 80 % (0.04 %), HlMeOHe (250 mg/kg per day) and V+HlMeOHe (250 mg/kg per day). Animals inhaled V twice a week. Blood samples were obtained at designated time points: 0, 24 and 72 hours; 7, 14 and 21 days. Subsequently, the samples were subjected to rigorous testing procedures, including the assessment of cell viability by fluorochromes, alkaline comet assay and micronucleus assay with acridine orange.
RESULTS: The inhalation of V, either as a standalone treatment or in conjunction with HlMeOHe, did not affect cell viability. Administration of HlMeOHe, did not result in the generation of single-strand breaks, a finding that contrast with the effects of V. The co-exposure of V and HlMeOHe exhibited from day 14-21 a significant decrease (p ≤ 0.05) in micronuclei assay, as well as a in single-strand breaks (p < 0.05) compared against control group.
CONCLUSION: These results suggest that HlMeOHe is not cytotoxic or genotoxic maybe because of its phenolic compounds, reducing the genotoxic effect induced by V.