Infection · 2026
GeneXpert CARBA-R assay for molecular characterization of carbapenem-resistance in Gram-negative bacteria in a tertiary care university hospital in Cairo-Egypt.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Shawky Heba, El Banna Afaf Mohamed Samy, AbdulRahman Eiman Mohammed, Mokhtar Ahmed, Balah Mariam Mahmoud
Аннотация
BACKGROUND: Carbapenem-resistant Enterobacterales are prevalent and increasingly reported in Egyptian hospitals. Carbapenemase production is the main mechanism of carbapenem resistance that carries the risk of dissemination among different bacterial species.
PURPOSE: the study aimed to detect the distribution of carbapenemase genes among Enterobacterales and Pseudomonas species isolated from clinical samples in a tertiary care university hospital in Cairo, Egypt.
METHODS: GeneXpert real time multiplex PCR was used for detection of carbapenemase genes in carbapenem-resistant isolates from patients admitted to Cairo University hospitals during the period from September 2021 to July 2023. Carbapenem-resistant isolates were identified by conventional antimicrobial susceptibility testing using the disc diffusion method. E. coli ATCC® 25922™ and Pseudomonas aeruginosa ATCC® 27853™ were used as quality control strains for disc diffusion. The isolates were tested for each of the following genes, blaKPC, blaNDM, blaOXA, blaVIM and blaIMP.
RESULTS: A total of 330 carbapenem-resistant isolates were identified. A single carbapenemase gene was detected in 106 (32.1%) of isolates, 211 (63.9%) had two genes compared to three genes in six (1.8%) of isolates. The most common resistance gene was blaNDM (90.3%) followed by blaOXA (68.5%).
CONCLUSION: The detection of carbapenemase genes using the GeneXpert Carba-R assay represents a significant advancement in combating antimicrobial resistance. This assay is easy and rapid; offering results within approximately 50 min; thus, represents significant added value to traditional culture methods, which require 48 h or more. Despite the advantages of GeneXpert, the assay has some limitations, as it targets only the five most common carbapenemase gene families, and may miss less common genes.
PURPOSE: the study aimed to detect the distribution of carbapenemase genes among Enterobacterales and Pseudomonas species isolated from clinical samples in a tertiary care university hospital in Cairo, Egypt.
METHODS: GeneXpert real time multiplex PCR was used for detection of carbapenemase genes in carbapenem-resistant isolates from patients admitted to Cairo University hospitals during the period from September 2021 to July 2023. Carbapenem-resistant isolates were identified by conventional antimicrobial susceptibility testing using the disc diffusion method. E. coli ATCC® 25922™ and Pseudomonas aeruginosa ATCC® 27853™ were used as quality control strains for disc diffusion. The isolates were tested for each of the following genes, blaKPC, blaNDM, blaOXA, blaVIM and blaIMP.
RESULTS: A total of 330 carbapenem-resistant isolates were identified. A single carbapenemase gene was detected in 106 (32.1%) of isolates, 211 (63.9%) had two genes compared to three genes in six (1.8%) of isolates. The most common resistance gene was blaNDM (90.3%) followed by blaOXA (68.5%).
CONCLUSION: The detection of carbapenemase genes using the GeneXpert Carba-R assay represents a significant advancement in combating antimicrobial resistance. This assay is easy and rapid; offering results within approximately 50 min; thus, represents significant added value to traditional culture methods, which require 48 h or more. Despite the advantages of GeneXpert, the assay has some limitations, as it targets only the five most common carbapenemase gene families, and may miss less common genes.