International journal of dermatology · 2009
The anti-condyloma acuminatum effects of interferon-inducible protein 10 in vitro.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Li Huijuan, Yi Tao, Zhao Shuyun, Chen Pin, Cheng Chenxian, Wei Yuquan, Zhao Xia
Аннотация
BACKGROUND: Interferon-inducible protein 10 (IP-10) has been reported to show an effective antiviral and antineoplastic role in various murine models.
AIM: To investigate the anti-condyloma acuminatum effect and human papillomavirus (HPV)-inhibiting efficacy of a recombinant plasmid encoding IP-10 in vitro.
METHODS: A recombinant plasmid DNA carrying IP-10 cDNA was constructed. Condyloma acuminatum tissue particles were transfected with IP-10 and examined for apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining and flow cytometry techniques. Relative real-time polymerase chain reaction (PCR) was used to validate the HPV inhibited level of the treatment groups. Immunohistochemistry was used to determine the IP-10 expression on condyloma acuminatum tissues.
RESULTS: Transfected IP-10 was expressed mainly in the cytoplasm of the condyloma acuminatum tissues. Plentiful apoptosis was observed in condyloma acuminatum tissues transfected with IP-10. In addition, HPV expression was lower in IP-10-treated tissues than in control tissues.
CONCLUSIONS: These observations suggest that IP-10 has strong anti-condyloma acuminatum effects, inducing apoptosis and inhibiting HPV, and therefore may be a novel and potentially effective therapy for condyloma acuminatum.
AIM: To investigate the anti-condyloma acuminatum effect and human papillomavirus (HPV)-inhibiting efficacy of a recombinant plasmid encoding IP-10 in vitro.
METHODS: A recombinant plasmid DNA carrying IP-10 cDNA was constructed. Condyloma acuminatum tissue particles were transfected with IP-10 and examined for apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining and flow cytometry techniques. Relative real-time polymerase chain reaction (PCR) was used to validate the HPV inhibited level of the treatment groups. Immunohistochemistry was used to determine the IP-10 expression on condyloma acuminatum tissues.
RESULTS: Transfected IP-10 was expressed mainly in the cytoplasm of the condyloma acuminatum tissues. Plentiful apoptosis was observed in condyloma acuminatum tissues transfected with IP-10. In addition, HPV expression was lower in IP-10-treated tissues than in control tissues.
CONCLUSIONS: These observations suggest that IP-10 has strong anti-condyloma acuminatum effects, inducing apoptosis and inhibiting HPV, and therefore may be a novel and potentially effective therapy for condyloma acuminatum.