Journal of occupational and environmental medicine · 2012
mRNA expression levels among cell regulatory and DNA damage genes in benzene-exposed workers in China.
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Wang Qi, Ye Rong, Ye Yun-Jie, Wan Jun-Xiang, Sun Pin, Zhu Yiliang, Au William, Xia Zhao-Lin
Аннотация
OBJECTIVE: We investigated how cells respond to the induction of DNA damage, focusing specifically on mRNA expression levels of cell regulatory and DNA repair genes under exposure to benzene.
METHOD: The study sample was classified into three groups: direct exposure to benzene (A), indirect exposure to benzene (B), and non-exposed (C). Concentrations of benzene in the air of workplaces were monitored. Further blood biochemical parameters, cell cycle-regulated and DNA damage-related genes expression were analyzed.
RESULTS: The mRNA expression levels of Apel, Rad51, Bcl-2, Bax, Xpa, and Xpc genes were significantly down-regulated in groups A and B, with a dramatic up-regulation of p21 gene in group A accompanied by significantly lower counts of white blood cells, hemoglobin, platelets and lymphocyte subsets of CD8+, CD4+, T and B cells.
CONCLUSION: The results indicated that exposure to benzene had significantly altered mRNA expression of some critical cell regulatory and DNA repair genes.
METHOD: The study sample was classified into three groups: direct exposure to benzene (A), indirect exposure to benzene (B), and non-exposed (C). Concentrations of benzene in the air of workplaces were monitored. Further blood biochemical parameters, cell cycle-regulated and DNA damage-related genes expression were analyzed.
RESULTS: The mRNA expression levels of Apel, Rad51, Bcl-2, Bax, Xpa, and Xpc genes were significantly down-regulated in groups A and B, with a dramatic up-regulation of p21 gene in group A accompanied by significantly lower counts of white blood cells, hemoglobin, platelets and lymphocyte subsets of CD8+, CD4+, T and B cells.
CONCLUSION: The results indicated that exposure to benzene had significantly altered mRNA expression of some critical cell regulatory and DNA repair genes.
Предметные рубрики MeSH
AdultAir Pollutants, OccupationalApelinBenzeneBiomarkersCell Cycle ProteinsChemical IndustryChinaCyclin-Dependent Kinase Inhibitor p21DNA RepairDNA-Binding ProteinsFemaleGene Expression RegulationHumansIntercellular Signaling Peptides and ProteinsMaleOccupational ExposureProto-Oncogene Proteins c-bcl-2RNA, MessengerRad51 RecombinaseReal-Time Polymerase Chain ReactionSurveys and QuestionnairesXeroderma Pigmentosum Group A Proteinbcl-2-Associated X Protein