Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih · 2006
Comparison between immunofluorescence and PCR in detecting human papilloma virus in condyloma acuminata.
Сравнительное исследованиеОценочное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
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Liu Fang, Wang Jia-Bi, Liu Yue-Hua, Zuo Ya-Gang, Man Xiao-Hong
Аннотация
OBJECTIVE: To compare the effectiveness of immunofluorescence and polymerase chain reaction (PCR) in detecting human papilloma virus (HPV) in condyloma acuminata (CA).
METHODS: HPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR, respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism (RFLP).
RESULTS: The positive detective rates of immunofluorescence and PCR were 56.67% (34/60) and 96.67% (58/ 60), respectively (P < 0.01). RFLP results showed HPV6 and HPV11 were the main subtypes in the detected virus, which accounted for 98.28%.
CONCLUSION: The sensibility of PCR is superior to that of immunofluorescence.
METHODS: HPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR, respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism (RFLP).
RESULTS: The positive detective rates of immunofluorescence and PCR were 56.67% (34/60) and 96.67% (58/ 60), respectively (P < 0.01). RFLP results showed HPV6 and HPV11 were the main subtypes in the detected virus, which accounted for 98.28%.
CONCLUSION: The sensibility of PCR is superior to that of immunofluorescence.