Медицинская библиотека · Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih · 2006

Comparison between immunofluorescence and PCR in detecting human papilloma virus in condyloma acuminata.

Liu Fang, Wang Jia-Bi, Liu Yue-Hua, Zuo Ya-Gang, Man Xiao-Hong

Сравнительное исследованиеОценочное исследованиеСтатьяПеревод в очереди

Аннотация

OBJECTIVE

To compare the effectiveness of immunofluorescence and polymerase chain reaction (PCR) in detecting human papilloma virus (HPV) in condyloma acuminata (CA).

METHODS

HPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR, respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism (RFLP).

RESULTS

The positive detective rates of immunofluorescence and PCR were 56.67% (34/60) and 96.67% (58/ 60), respectively (P < 0.01). RFLP results showed HPV6 and HPV11 were the main subtypes in the detected virus, which accounted for 98.28%.

CONCLUSION

The sensibility of PCR is superior to that of immunofluorescence.

Для цитирования: Liu Fang, Wang Jia-Bi, Liu Yue-Hua, Zuo Ya-Gang, Man Xiao-Hong. Comparison between immunofluorescence and PCR in detecting human papilloma virus in condyloma acuminata.. Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih. 2006.

Предметные рубрики MeSH

Condylomata Acuminata Female Human papillomavirus 11 Human papillomavirus 6 Humans Male Microscopy, Fluorescence Polymerase Chain Reaction

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