Evaluation of anti-oxidant and anticancer effect of marine algae Cladophora glomerata in HT29 colon cancer cell lines- an in-vitro study.

BACKGROUND

Marine algae are a huge Pandora's box of rich nutrients and huge medicinal compounds. These therapeutic compounds are investigated widely for their anticancer, antioxidant, and anti-diabetic properties.

OBJECTIVES

This study aims to evaluate the antioxidant and anticancer effect of the marine algae Cladophora glomerata (collected from Hare Island-Tuticorin region) on HT-29 colon cancer cell lines.

METHODOLOGY: The marine algae, Cladophora glomerata, was collected, processed, and authenticated. Methanol, Ethyl acetate, Chloroform, and Hexane extracts were prepared using a hot solvent extraction process. These extracts were subjected to SOD assay and MTT assay. 5 Fluorouracil was used as the positive control.

RESULTS

The antioxidant activity of the SOD assay was found to be 85.66±0.81, 80.10±1.25, and 98±0.93 U/mg protein for methanol, ethyl acetate, chloroform, and hexane extracts, respectively. L-Ascorbic acid was used as the positive control whose SOD antioxidant value was found to be 139±1.24 U/mg protein. The IC50 value of methanol, ethyl acetate, chloroform, hexane algae extracts, and 5 Fluorouracil against HT29 cell lines was calculated to be 28.46±0.65, 48.56±1.19, 93.7±0.91, 88.53±0.83, and 8.2±1.3 μg/ml, respectively.

CONCLUSION

From the above study, we can infer that the methanol extracts of the algae Cladophora glomerata have excellent anticancer activity. Therefore, these compounds can be purified and analyzed further for a potential lead as an anticancer molecule.