Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 1998
Immunoglobulin E-mediated anaphylaxis activates nuclear factor kappaB in rat small intestine.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Carvalho Tavares J, Bayón Y, Sánchez Crespo M
Аннотация
OBJECTIVE AND DESIGN: Since IgE-dependent reactions induce the inducible isoform of NO synthase, we postulated the involvement of the transcription factor NF-kappaB.
MATERIALS: 72 Wistar rats were divided into 6 groups and used to study the hemorrhagic necrosis of the small intestine elicited by anaphylaxis.
TREATMENT: Passive anaphylaxis was produced by i.p. sensitization with IgE anti-dinitrophenyl monoclonal antibody and i.v. challenge with the cognate antigen.
METHODS: Competitive PCR was used to assay the expression of p50 subunit of NF-kappaB. kappaB-binding activity was assayed by electrophoretic mobility shift assay.
RESULTS: The PCR assay showed a time-dependent increase of mRNA coding for the p50 subunit of NF-kappaB, which was maximal 1 h after challenge (40+/-3, versus 230+/-32 fM, mean +/- SEM) and decreased to prechallenge level at 4h. kappaB-binding activity was also increased.
CONCLUSIONS: IgE-mediated reactions trigger a pathway for nuclear signaling that seems to be related to the intermediate/late response of the allergic reaction.
MATERIALS: 72 Wistar rats were divided into 6 groups and used to study the hemorrhagic necrosis of the small intestine elicited by anaphylaxis.
TREATMENT: Passive anaphylaxis was produced by i.p. sensitization with IgE anti-dinitrophenyl monoclonal antibody and i.v. challenge with the cognate antigen.
METHODS: Competitive PCR was used to assay the expression of p50 subunit of NF-kappaB. kappaB-binding activity was assayed by electrophoretic mobility shift assay.
RESULTS: The PCR assay showed a time-dependent increase of mRNA coding for the p50 subunit of NF-kappaB, which was maximal 1 h after challenge (40+/-3, versus 230+/-32 fM, mean +/- SEM) and decreased to prechallenge level at 4h. kappaB-binding activity was also increased.
CONCLUSIONS: IgE-mediated reactions trigger a pathway for nuclear signaling that seems to be related to the intermediate/late response of the allergic reaction.
Предметные рубрики MeSH
AnaphylaxisAnimalsAntibodies, MonoclonalBinding, CompetitiveDinitrophenolsGene ExpressionGene Expression RegulationGlyceraldehyde-3-Phosphate DehydrogenasesImmunoglobulin EIntestine, SmallKineticsMaleNF-kappa BNitric Oxide SynthaseNitric Oxide Synthase Type IIPolymerase Chain ReactionRNA, MessengerRatsRats, WistarSerum Albumin, Bovine