Journal of global antimicrobial resistance · 2022
Draft genome sequence of a multidrug-resistant Campylobacter coli ST825 carrying several acquired antibiotic resistance genes isolated from poultry in China.
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Wu Lin, Zhai Xia, Jin Yaojian, Chen Haohao, Huang Junwei
Аннотация
OBJECTIVES: Campylobacter coli is a typical food-borne pathogen worldwide known to cause bacterial gastroenteritis in humans. This study reported a draft whole genome sequence of C. coli isolate obtained from the caecal contents of poultry in Jinhua, China.
METHODS: Whole genomic DNA was sequenced using an Illumina Novaseq 6000 platform in 150 bp paired-end mode. The generated reads were de novo assembled by SPAdes v.3.12.0. All probable coding sequences were annotated using the RAST (Rapid Annotation using Subsystem Technology), and antibiotic resistance-related genes were also further identified by ResFinder 4.1 and rgi 5.1.1.
RESULTS: The draft genome contained 1 794 608 bp, a total of 69 contigs, belonging to sequence type (ST) ST825, comprising 1972 coding genes, 42 transfer RNAs, 2 ribosomal RNA, and with a GC content of 31.2%. The RAST analysis revealed a total of 698 subsystems in the genome of C. coli WL32 strain, with most of the genes associated with amino acids and derivatives (21.35%) and protein metabolism (17.05%). The genes related to antibiotic resistance, including erm(B) gene associated with macrolide resistance, blaOXA-61 gene associated with resistance to β-lactams, aac (6')-aph(2'), ant(6)-Ia, aph(2')-If, aph(3')-III gene associated with resistance to aminoglycosides, tetO gene associated with resistance to tetracycline, cat gene associated with amphenicol, and gyrA with fluoroquinolone Thr-86-Ile substitution, were identified. Also, the virulence genes, including motA, motB, flaG, fliE, fliF, fliG, flhB, and flhF genes, were identified by WGS analysis.
CONCLUSION: We report the draft genome sequence of C. coli ST825 isolate obtained from a poultry in China, which could provide potential information for tracking the potential spread of such a multidrug-resistant clone from poultry product processing to human beings.
METHODS: Whole genomic DNA was sequenced using an Illumina Novaseq 6000 platform in 150 bp paired-end mode. The generated reads were de novo assembled by SPAdes v.3.12.0. All probable coding sequences were annotated using the RAST (Rapid Annotation using Subsystem Technology), and antibiotic resistance-related genes were also further identified by ResFinder 4.1 and rgi 5.1.1.
RESULTS: The draft genome contained 1 794 608 bp, a total of 69 contigs, belonging to sequence type (ST) ST825, comprising 1972 coding genes, 42 transfer RNAs, 2 ribosomal RNA, and with a GC content of 31.2%. The RAST analysis revealed a total of 698 subsystems in the genome of C. coli WL32 strain, with most of the genes associated with amino acids and derivatives (21.35%) and protein metabolism (17.05%). The genes related to antibiotic resistance, including erm(B) gene associated with macrolide resistance, blaOXA-61 gene associated with resistance to β-lactams, aac (6')-aph(2'), ant(6)-Ia, aph(2')-If, aph(3')-III gene associated with resistance to aminoglycosides, tetO gene associated with resistance to tetracycline, cat gene associated with amphenicol, and gyrA with fluoroquinolone Thr-86-Ile substitution, were identified. Also, the virulence genes, including motA, motB, flaG, fliE, fliF, fliG, flhB, and flhF genes, were identified by WGS analysis.
CONCLUSION: We report the draft genome sequence of C. coli ST825 isolate obtained from a poultry in China, which could provide potential information for tracking the potential spread of such a multidrug-resistant clone from poultry product processing to human beings.