Platelets · 2020
Search for SARS-CoV-2 RNA in platelets from COVID-19 patients.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Bury Loredana, Camilloni Barbara, Castronari Roberto, Piselli Elisa, Malvestiti Marco, Borghi Mariachiara, KuchiBotla Haripriya, Falcinelli Emanuela, Petito Eleonora, Amato Felice, Paliani Ugo, Vaudo Gaetano, Cerotto Vittorio, Gori Fabio, Becattini Cecilia, De Robertis Edoardo, Lazzarini Teseo, Castaldo Giuseppe, Mencacci Antonella, Gresele Paolo
Аннотация
The frequent finding of thrombocytopenia in patients with severe SARS-CoV-2 infection (COVID-19) and previous evidence that several viruses enter platelets suggest that SARS-CoV-2 might be internalized by platelets of COVID-19. Aim of our study was to assess the presence of SARS-CoV-2 RNA in platelets from hospitalized patients with aconfirmed diagnosis of COVID-19. RNA was extracted from platelets, leukocytes and serum from 24 COVID-19 patients and 3 healthy controls, real-time PCR and ddPCR for viral genes were carried out. SARS-CoV-2 RNA was not detected in any of the samples analyzed nor in healthy controls, by either RT-PCR or ddPCR, while RNA samples from nasopharyngeal swabs of COVID-19 patients were correctly identified. Viral RNA was not detected independently of viral load, of positive nasopharyngeal swabs, or viremia, the last detected in only one patient (4.1%). SARS-CoV-2 entry in platelets is not acommon phenomenon in COVID-19 patients, differently from other viral infections.