Effects of cyanogenic agents on reproduction and neonatal development in rats.

A diet consisting of 50% gari (a form of cassava meal) was found to cause no significant biochemical and haematological changes in adult female rats. Although this diet caused a significant increase in their serum thiocyanate levels, there was no corresponding significant increase in the weight of their thyroid glands. The offspring of these rats had significantly lower birth weights and brain weights and never attained the same adult weights as those of the controls. Adult female rats fed a diet consisting entirely of raw cassava had significantly reduced haematological and biochemical indices. This diet also caused an increased incidence of cannibalism and a significant reduction in the frequency of pregnancy, the average number of the litter and the birth weights among these rats. In addition there was an increased incidence of neonatal deaths among the offspring which also had poor development, reduced brain weights and an increased tendency towards biting their litter-mates. Adult female rats fed diets containing 5 and 10 g cyanide/100 g laboratory diet survived for more than 3 months but never became pregnant. They developed enlarged thyroid glands and tumours of the large intestine. The relevance of these findings to the areas of the world where cyanogenic glycosides form the staple diet is discussed.