Fertility and sterility · 2016
Integrating insulin into single-step culture medium regulates human embryo development in vitro.
СтатьяМногоцентровое исследованиеРандомизированное исследованиеНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Fawzy Mohamed, Sabry Mohamed, Nour Mohamed, Abdelrahman Mohamed Y, Roshdy Eman, Magdi Yasmin, Abdelghafar Hazem
Аннотация
OBJECTIVE: To evaluate the effect of supplementing single-step embryo culture medium with insulin on human embryo development.
DESIGN: Comparative study.
SETTING: Two private centers.
PATIENT(S): The study involved a sibling oocyte split of 5,142 retrieved oocytes from 360 patients.
INTERVENTION(S): Sibling oocytes split after intracytoplasmic sperm injection for culture from day 0 through day 5 or 6 in insulin-supplemented or control medium. Women were split to receive their embryos from insulin-supplemented or control medium.
MAIN OUTCOME MEASURE(S): Clinical pregnancy rate.
RESULT(S): There were significantly higher rates of clinical, ongoing, and twin pregnancies in the insulin-supplemented arm than in the control arm. On day 3, embryo quality and compaction were higher in insulin-supplemented medium. On day 5, insulin supplementation showed higher rates of blastocyst formation, quality, and cryopreservation.
CONCLUSION(S): Insulin supplementation of single-step embryo culture medium from day 0 through day 5 or 6 improved clinical pregnancy rate and human embryo development. However, these findings need further confirmation through a multicenter randomized controlled trial that may include other patient populations and different culture media.
DESIGN: Comparative study.
SETTING: Two private centers.
PATIENT(S): The study involved a sibling oocyte split of 5,142 retrieved oocytes from 360 patients.
INTERVENTION(S): Sibling oocytes split after intracytoplasmic sperm injection for culture from day 0 through day 5 or 6 in insulin-supplemented or control medium. Women were split to receive their embryos from insulin-supplemented or control medium.
MAIN OUTCOME MEASURE(S): Clinical pregnancy rate.
RESULT(S): There were significantly higher rates of clinical, ongoing, and twin pregnancies in the insulin-supplemented arm than in the control arm. On day 3, embryo quality and compaction were higher in insulin-supplemented medium. On day 5, insulin supplementation showed higher rates of blastocyst formation, quality, and cryopreservation.
CONCLUSION(S): Insulin supplementation of single-step embryo culture medium from day 0 through day 5 or 6 improved clinical pregnancy rate and human embryo development. However, these findings need further confirmation through a multicenter randomized controlled trial that may include other patient populations and different culture media.
Предметные рубрики MeSH
AdolescentAdultBlastocystCryopreservationCulture MediaEgyptEmbryo Culture TechniquesEmbryo TransferEmbryonic DevelopmentFemaleFertilityFertility Agents, FemaleHumansInfertilityInsulinMaleOocyte RetrievalPregnancyPregnancy RatePregnancy, TwinProspective StudiesSperm Injections, IntracytoplasmicTime FactorsTreatment OutcomeYoung Adult