Transfusion · 2025
Evaluating the quality of X-ray irradiated deglycerolized red blood cells stored in saline-adenosine-glucose-mannitol additive solution.
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Winter Kelly M, Webb Rachel G, Mazur Eugenia, Dennington Peta M, von Wielligh Kobie, Marks Denese C
Аннотация
BACKGROUND: Rare phenotype red blood cells (RBCs) are routinely cryopreserved and stored at -80°C for over 10 years. Upon thawing and deglycerolization, RBCs resuspended in saline-adenosine-glucose-mannitol (SAGM) have a limited 24-h shelf-life. There is a lack of international consensus as to whether irradiation of deglycerolized RBCs is necessary for patients at risk of transfusion-associated graft-versus-host disease. The aim of this study was to evaluate the quality of X-ray irradiated deglycerolized RBCs resuspended in SAGM.
STUDY DESIGN AND METHODS: Matched RBC components (n = 21 pairs) were glycerolized with 40% glycerol, frozen at -80°C, deglycerolized using an ACP-215 cell washer, and resuspended in SAGM. One of each pair was X-ray irradiated post-deglycerolization, while the other remained untreated (control). RBCs were sampled pre-freeze and immediately, 24-, 48-, and 72-h post-irradiation. Quality indicators including RBC indices, metabolic, and biochemical parameters were measured.
RESULTS: There were no significant differences between X-irradiated and control groups for volume, hemoglobin (Hb), hematocrit, or supernatant Hb immediately post-thaw. Hemolysis was higher in X-irradiated deglycerolized RBCs (0.35 ± 0.14%) than controls (0.32 ± 0.05%) after 24-h (p = 0.077). Potassium (K+) release was significantly higher in X-irradiated RBCs compared to controls after 24-h (6.26 ± 0.95 mmol/unit vs. 3.21 ± 1.01 mmol/unit respectively; p < .001).
DISCUSSION: X-irradiation had a significant effect on K+ release from deglycerolized RBCs during storage, and to a lesser extent, hemolysis. The K+ and hemolysis levels at 24 h post-irradiation were acceptable, as were the other quality parameters, indicating that X-irradiation of these components is feasible.
STUDY DESIGN AND METHODS: Matched RBC components (n = 21 pairs) were glycerolized with 40% glycerol, frozen at -80°C, deglycerolized using an ACP-215 cell washer, and resuspended in SAGM. One of each pair was X-ray irradiated post-deglycerolization, while the other remained untreated (control). RBCs were sampled pre-freeze and immediately, 24-, 48-, and 72-h post-irradiation. Quality indicators including RBC indices, metabolic, and biochemical parameters were measured.
RESULTS: There were no significant differences between X-irradiated and control groups for volume, hemoglobin (Hb), hematocrit, or supernatant Hb immediately post-thaw. Hemolysis was higher in X-irradiated deglycerolized RBCs (0.35 ± 0.14%) than controls (0.32 ± 0.05%) after 24-h (p = 0.077). Potassium (K+) release was significantly higher in X-irradiated RBCs compared to controls after 24-h (6.26 ± 0.95 mmol/unit vs. 3.21 ± 1.01 mmol/unit respectively; p < .001).
DISCUSSION: X-irradiation had a significant effect on K+ release from deglycerolized RBCs during storage, and to a lesser extent, hemolysis. The K+ and hemolysis levels at 24 h post-irradiation were acceptable, as were the other quality parameters, indicating that X-irradiation of these components is feasible.