Dataset on the influence of carbon dioxide (CO2) concentration and growth temperature on biomass productivity of chlorella micro-algae in green fuel synthesis.

Chlorella micro-algae species are widely employed in sustainable biofuel production and development due to their high biomass and lipid accumulation rates. In this data article, the effect of carbon dioxide (CO2) concentration and culture temperature on the growth rate and biomass productivity of chlorella vulgaris micro algae in sustainable biofuel development was investigated. The micro-algae strains (SAG 211-11b) were isolated from the International Institute of Tropical Agriculture (IITA) and cultured with modified blue green algae (Modified BG-11) nutrients under photoautotrophic conditions and continuous illumination. The light intensity was maintained at 300μmol photons m⁻² s⁻¹ and measured using a portable quantum light meter (Model 3415F, pulse photoelectric sensor; spectrum Technologies Inc., USA). The pre-cultured micro-algal cells were transferred into bubble bioreactors consisting of 500 mL Erlenmeyer flasks that contain 300 mL of BG-11 medium. The CO₂ concentration in the aeration gas was adjusted to 0.04% (air), 2%, 4%, 6%, 8%, 10%, 12%, and 15%, and experiments were conducted at growth temperatures of 25°C, 27.5°C, 30°C and 32.5°C. The experimental measurements were analyzed with statistical tools (standard error and analysis of variance, ANOVA) by using IBM SPSS statistics version 30.0. The air (0.04% CO2 concentration) has the lowest biomass productivity at all the tested growth temperatures. While the highest biomass productivity of 3.75± 0.0927g L⁻¹ day⁻¹ was recorded at growth temperature of 30°C and under optimum CO2 concentration of 6.0%. However, further increases in growth temperature beyond this optimum level (30°C) resulted in a sharp decline in growth rate and biomass yield. The null hypothesis (H0) was rejected at the 5% significance value since the calculated FRstatistics > Fcritical at α = 0.05. Finally, the data is of utmost importance to the bio-energy researchers and scientific community in selecting optimum growth parameters for enhanced micro-algae growth and biomass productivity.