Effect of Deoxyglucose on Cultured Glial Cells of the Rat Cerebral Cortex under Conditions of LPS Stimulation.

It was shown that blocking of glycolysis by 2-deoxy-D-glucose (DG) inhibits LPS-induced NO production in glial cell cultures and reduces the mitochondrial membrane potential. Lactate partially prevented the decrease in the mitochondrial membrane potential of gliocytes, but did not affect the inhibitory effect of DG. Immunocytochemical typing showed the presence of a large number of astrocytes and microglia in the culture. In the control, microglial cells had a large number of processes, which is typical of nonactivated cells, whereas in LPS-treated cultures, microglia had a flattened amoeboid morphology typical of activated microglia. In cultures treated with LPS against the background of DG, both cells with processes and amoeboid cells were present. Our results indicate that inhibition of glycolysis is a strong modulating factor in inflammation.