Journal of basic and clinical physiology and pharmacology · 2020
In vitro antioxidants and hepatoprotective effects of Pleurotus tuber-regium on carbon tetrachloride-treated rats.
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Okolo Kenneth O, Orisakwe Orish E
Аннотация
OBJECTIVES: This study has characterized the phytoconstituents and evaluated the in vitro antioxidant and hepatoprotective effects of Pleurotus tuber-regium induced by carbon tetrachloride (CCl4). In vitro antioxidant assay of ethanol extract of P. tuber-regium and gas chromatography-mass spectrometry analyses to identify the phytoconstituents were carried out.
METHODS: Sixty rats were divided into six groups of 10 animals in each group and treated as follows for 13 weeks. Group I (control) received 3 mL/kg olive oil intraperitoneal twice weekly in addition to feed and water ad libitum. Group II received CCl4 3 mL/kg twice weekly. Groups III, IV and V received 100, 200 and 500 mg/kg wild edible P. tuber-regium mixed with feed by ingestion daily in addition to 3 mL/kg CCl4 twice weekly, respectively. Group VI received 500 mg P. tuber-regium daily. Liver and body weights were recorded. Liver function tests, oxidative stress biomarkers, bilirubin, ascorbic acid and α-tocopherol were assayed. Histopathology of the liver was carried out. The gas chromatography-mass spectroscopy analysis yielded 10 antioxidants.
RESULTS: CCl4 increased the levels of alanine transaminase, aspartate transaminase, alkaline phosphatase, bilirubin and malondialdehyde from 24±1.778 iu/L, 53±3.7 iu/L, 257±19 iu/L, 0.45±0.03 mg/dL and 1.90±0.42 μmol/L in the control group to 48±2.5 iu/L, 81±2.10 iu/L, 495±38 iu/L, 1.20±0.09 mg/dL and 14.0±2.6 μmol/L in the treated group, respectively.
CONCLUSIONS: P. tuber-regium prevented the necrosis, edema and vein congestion observed in the CCl4-only group. P. tuber-regium is effective in protecting the liver against CCl4-induced damage.
METHODS: Sixty rats were divided into six groups of 10 animals in each group and treated as follows for 13 weeks. Group I (control) received 3 mL/kg olive oil intraperitoneal twice weekly in addition to feed and water ad libitum. Group II received CCl4 3 mL/kg twice weekly. Groups III, IV and V received 100, 200 and 500 mg/kg wild edible P. tuber-regium mixed with feed by ingestion daily in addition to 3 mL/kg CCl4 twice weekly, respectively. Group VI received 500 mg P. tuber-regium daily. Liver and body weights were recorded. Liver function tests, oxidative stress biomarkers, bilirubin, ascorbic acid and α-tocopherol were assayed. Histopathology of the liver was carried out. The gas chromatography-mass spectroscopy analysis yielded 10 antioxidants.
RESULTS: CCl4 increased the levels of alanine transaminase, aspartate transaminase, alkaline phosphatase, bilirubin and malondialdehyde from 24±1.778 iu/L, 53±3.7 iu/L, 257±19 iu/L, 0.45±0.03 mg/dL and 1.90±0.42 μmol/L in the control group to 48±2.5 iu/L, 81±2.10 iu/L, 495±38 iu/L, 1.20±0.09 mg/dL and 14.0±2.6 μmol/L in the treated group, respectively.
CONCLUSIONS: P. tuber-regium prevented the necrosis, edema and vein congestion observed in the CCl4-only group. P. tuber-regium is effective in protecting the liver against CCl4-induced damage.