Thermally reversible Diels-Alder linkers enable reusable methacrylate supports for enzyme immobilisation.

Spent enzyme immobilisation resins constitute microplastic waste. Here, commercially available C2-amine methacrylate beads are converted into reusable supports through furan decoration and a thermally cleavable Diels-Alder linker bearing an NHS ester. The enzyme-linker assembly is removed by retro-Diels-Alder reaction, regenerating the support over multiple cycles.