International immunopharmacology · 2022
Pellino2 accelerate inflammation and pyroptosis via the ubiquitination and activation of NLRP3 inflammation in model of pediatric pneumonia.
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Liu Xiaqing, Lin Zhengfang, Yin Xiaoli
Аннотация
BACKGROUND: Mycoplasma pneumoniae pneumonia (MPP) is a common and frequently-occurring disease in pediatrics. This study aims to via unveiling the novel effects and mechanisms of Pellino2 in model of pediatric pneumonia.
MATERIALS AND METHODS: Male infancy C57BL/6 mice were injected with 2 mg/kg of LPS (Sigma-Aldrich Merck KGaA). THP-1 cells were induced with LPS and ATP.
RESULTS: The expression of Pellino2 mRNA and protein in patients with pediatric pneumonia or mice with pediatric pneumonia were reduced. Pellino2 accelerated lung injury and expanded inflammation and pyroptosis in lung tissue of pediatric pneumonia in vivo and vitro model. Furthermore, the inhibition of Pellino2 reduced lung injury and weakened inflammation and pyroptosis in lung tissue of pediatric pneumonia in vivo and vitro model. Pellino2 protein catenated NLRP3 protein, and Pellino2 promoted ubiquitination and activation of NLRP3 inflammation in model of pediatric pneumonia. Pellino2 accelerate inflammation and pyroptosis in model of pediatric pneumonia by NLRP3.
CONCLUSIONS: These results suggest that Pellino2 accelerate inflammation and pyroptosis via the induction of ubiquitination and activation of NLRP3 inflammation in model of pediatric pneumonia, Pellino2 may serve as a potential approach for the treatment of pediatric pneumonia and other inflammatory diseases.
MATERIALS AND METHODS: Male infancy C57BL/6 mice were injected with 2 mg/kg of LPS (Sigma-Aldrich Merck KGaA). THP-1 cells were induced with LPS and ATP.
RESULTS: The expression of Pellino2 mRNA and protein in patients with pediatric pneumonia or mice with pediatric pneumonia were reduced. Pellino2 accelerated lung injury and expanded inflammation and pyroptosis in lung tissue of pediatric pneumonia in vivo and vitro model. Furthermore, the inhibition of Pellino2 reduced lung injury and weakened inflammation and pyroptosis in lung tissue of pediatric pneumonia in vivo and vitro model. Pellino2 protein catenated NLRP3 protein, and Pellino2 promoted ubiquitination and activation of NLRP3 inflammation in model of pediatric pneumonia. Pellino2 accelerate inflammation and pyroptosis in model of pediatric pneumonia by NLRP3.
CONCLUSIONS: These results suggest that Pellino2 accelerate inflammation and pyroptosis via the induction of ubiquitination and activation of NLRP3 inflammation in model of pediatric pneumonia, Pellino2 may serve as a potential approach for the treatment of pediatric pneumonia and other inflammatory diseases.