A Facile Dispersive Solid Phase Extraction of Vancomycin From Plasma and Deionized Water Samples Using UiO-66-NH2 Metal-Organic Framework Prior to HPLC-MS/MS Determination.

A simple, accurate, cost-effective, and sensitive analysis method for the determination of vancomycin in plasma samples has been reported using high-performance liquid chromatography-tandem mass spectrometry. UiO-66-NH2 metal-organic framework was synthesized through a hydrothermal method, 5 mg of this compound was introduced into 5 mL of model solution or pretreated plasma adjusted to a pH of 8. The mixture was vortexed for 1 min and then the sorbent particles were separated by centrifugation. The upper phase of sorbents was removed and the analytes adsorbed were eluted by a 250 µL mixture of deionized water (pH 2) and methanol at a 1:1 ratio, v/v, under vortexing for 4 min from the sorbent surface. After centrifugation, the eluent was analyzed by the determination system. The synthesized nanoparticles underwent characterization using X-ray diffraction, Fourier transform infrared spectrometry, and scanning electron microscopy. To achieve optimal efficacy, optimization was carried out at every stage of the project, encompassing adsorption, extraction, and desorption processes. Approved validation data consisting of method detection limit (22 and 225 ng/mL in deionized water and plasma, respectively) and limit of quantification (73 and 774 ng/mL in deionized water and plasma, respectively), a wide linear range of the calibration curve (73-250 ng/mL and 774-1250 ng/mL in deionized water and plasma, respectively), and low relative standard deviations for interday and intraday precisions (≤ 4.8%) were obtained by the method. The proposed method can be successfully applied in the analysis for the determination of vancomycin in plasma and deionized water samples.