Optimization of Lipid Extraction from Spirulina spp. by Ultrasound Application and Mechanical Stirring Using the Taguchi Method of Experimental Design.

The present study uses the Taguchi method of experimental design to optimize lipid extraction from Spirulina spp. by ultrasound application and mechanical stirring. A Taguchi L9 orthogonal array was used to optimize various parameters, such as methanol: chloroform (M:C) ratio, biomass: solvent ratio, and extraction time for lipid extraction. The results were analyzed using the signal-to-noise (S/N) ratio and analysis of variance (ANOVA). The biomass: solvent ratio significantly influenced lipid content (p < 0.05) with 92.1% and 92.3% contributions to the lipid and S/N ratio data, respectively. The extraction time presented a contribution value of 5.0%, while the M:C ratio presented the most negligible contribution of 0.4% for S/N data. The optimum extraction conditions were: M:C ratio of 1:1, biomass: solvent ratio of 1:60, and extraction time of 30 min. The predominant fatty acids were palmitic acid (44.5%), linoleic acid (14.9%), and gamma-linolenic acid (13.4%). The confirmation experiments indicated a lipid content of 8.7%, within a 95% confidence interval, proving the Taguchi method’s effectiveness in optimizing the process parameters for lipid extraction.