Wei sheng yan jiu = Journal of hygiene research · 2005
[Research on differential display genes of tolerant-damage induced by trichloroethylene].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Wei Qin-Zhi, Zhuang Zhi-Xiong, Yuan Jian-Hui, Huang Hai-Yan, Li Xiyi, Yang Xiaohua, Geng Lei
Аннотация
OBJECTIVE: To observe the differentially expressed genes of the human normal liver cell (L-02) induced by low concentration of trichloroethylene (TCE).
METHODS: The dose-effect relation of TCE toxicity was analyzed by means of MTT. 5 micromol/L was chosen as low concentration while 40 micromol/L as high concentration. Then L-02 were treated with low concentration, high concentration, pretreated with low concentration then attacked with high concentration of TCE respectively. Fluorescence differential display polymerase chain reaction (Fluoro DD-PCR) was used to search differentially expressed genes of the different treatment groups of TCE. Results 51 differential expressed strap were found, 11 differential straps have been cloned and sequenced. 9 of them were known gene, while 2 of them were new genes.
CONCLUSION: The differential straps would be identified to provide scientific base for research deeply the mechanism of low concentration TCE inducing adaptive response of L-02.
METHODS: The dose-effect relation of TCE toxicity was analyzed by means of MTT. 5 micromol/L was chosen as low concentration while 40 micromol/L as high concentration. Then L-02 were treated with low concentration, high concentration, pretreated with low concentration then attacked with high concentration of TCE respectively. Fluorescence differential display polymerase chain reaction (Fluoro DD-PCR) was used to search differentially expressed genes of the different treatment groups of TCE. Results 51 differential expressed strap were found, 11 differential straps have been cloned and sequenced. 9 of them were known gene, while 2 of them were new genes.
CONCLUSION: The differential straps would be identified to provide scientific base for research deeply the mechanism of low concentration TCE inducing adaptive response of L-02.