Synthesis of a new acetylcholinesterase-activated curcumin-derived fluores cent probe for highly sensitive and sensitive detection of diazinon pesticide and its application in foodstuffs and biological systems.

The extensive usage of diazinon in agriculture production has constituted a serious risk to food safety and public health. In this context, a new boron difluoride complex of curcumin was constructed as an effective enzyme-activatable fluorescent probe YPC. Acetylcholinesterase (AChE) is able to respond and break the unilateral ester bond on the dimethylcarbamate group of the probe YPC, which resulted in a significant enhancement effect orange fluorescence signal at 611 nm. Because diazinon has an obvious inhibitory effect on AChE activity, the fluorescence emission of probe YPC was quenched in the presence of diazinon, enabling it to be used as an excellent fluorescent platform for diazinon detection. More critically, the probe YPC has been successfully applied to the quantitative determination of trace residues of diazinon in agricultural product, and the determination results have high accuracy. Furthermore, the probe YPC could realize the detection of diazinon in living cells. Through this study, a novel detection technique will be established that can determine OPs residues in food and biological samples with high sensitivity and accuracy.